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. 2018 Jan 26;13(1):e0191964. doi: 10.1371/journal.pone.0191964

Table 3. Physical, mechanical and geometric properties of tibia obtained from 42-day-old broilers in control groups and supplemented with organic Zn in 25% of recommended dose with or without phytase inclusion.

Item Group Pooled SEM P-value
NC
(n = 10)
Zn25%
(n = 10)
Zn25%+phyt
(n = 10)
PC
(n = 10)
Bone general properties
Bone weight, g 22.8 21.1 25.1 23.5 0.8 ns
Bone length, mm 111 112 111 111 1 ns
Bone weight/bone length, g·mm-1 0.199ab 0.184a 0.224bc 0.235c 0.007 <0.001
Bone weight/body weight, % 1.12 1.04 1.02 0.94 0.05 ns
Bone geometrical properties
Horizontal internal diameter, mm 4.67a 5.81b 5.91b 5.68b 0.17 <0.001
Horizontal external diameter, mm 8.32a 9.49b 10.47c 9.82bc 0.28 <0.001
Vertical internal diameter, mm 4.29a 4.90b 5.29b 4.89b 0.14 <0.001
Vertical external diameter, mm 6.32a 8.12b 9.14c 8.24b 0.29 <0.001
Cross section area, mm2 26.3a 38.0b 50.5c 42.3bc 2.7 <0.001
Mean relative wall thickness, 0.67 0.65 0.75 0.71 0.03 ns
Cortical index, % 38.9a 38.9a 42.9b 41.2ab 0.75 0.009
Vertical cortical index, % 32.1a 39.7b 42.1b 40.4b 1.3 <0.001
Vertical wall thickness, mm 1.01a 1.61b 1.92c 1.67bc 0.09 <0.001
Midshaft volume, cm3 1.17a 1.71b 2.22c 1.69b 0.12 <0.001
Moment of inertia, mm4 86.4a 214.2b 358.3c 256.7b 30.6 <0.001
Index of gyration, mm 1.81a 2.38b 2.63c 2.38b 0.09 <0.001
Bone mechanical properties
Yield strength, N 163a 357c 293b 197a 21 <0.001
Ultimate strength, N 240a 441b 464b 289a 25 <0.001
Elastic stress, MPa 68.7b 75.6b 44.3a 33.6a 4.8 <0.001
Ultimate stress, MPa 100.9b 93.5b 71.0a 51.1a 6.0 0.001
Bending moment, N·m 1.81a 3.99c 3.27b 1.97a 0.25 <0.001

a, b, c–mean values in rows with different letters differ significantly at P<0.05; Data given are LSMeans; SEM–standard error of the mean

The description of the groups as in Table 1.