Table 3. Physical, mechanical and geometric properties of tibia obtained from 42-day-old broilers in control groups and supplemented with organic Zn in 25% of recommended dose with or without phytase inclusion.
Item | Group | Pooled SEM | P-value | ||||
---|---|---|---|---|---|---|---|
NC (n = 10) |
Zn25% (n = 10) |
Zn25%+phyt (n = 10) |
PC (n = 10) |
||||
Bone general properties | |||||||
Bone weight, g | 22.8 | 21.1 | 25.1 | 23.5 | 0.8 | ns | |
Bone length, mm | 111 | 112 | 111 | 111 | 1 | ns | |
Bone weight/bone length, g·mm-1 | 0.199ab | 0.184a | 0.224bc | 0.235c | 0.007 | <0.001 | |
Bone weight/body weight, % | 1.12 | 1.04 | 1.02 | 0.94 | 0.05 | ns | |
Bone geometrical properties | |||||||
Horizontal internal diameter, mm | 4.67a | 5.81b | 5.91b | 5.68b | 0.17 | <0.001 | |
Horizontal external diameter, mm | 8.32a | 9.49b | 10.47c | 9.82bc | 0.28 | <0.001 | |
Vertical internal diameter, mm | 4.29a | 4.90b | 5.29b | 4.89b | 0.14 | <0.001 | |
Vertical external diameter, mm | 6.32a | 8.12b | 9.14c | 8.24b | 0.29 | <0.001 | |
Cross section area, mm2 | 26.3a | 38.0b | 50.5c | 42.3bc | 2.7 | <0.001 | |
Mean relative wall thickness, | 0.67 | 0.65 | 0.75 | 0.71 | 0.03 | ns | |
Cortical index, % | 38.9a | 38.9a | 42.9b | 41.2ab | 0.75 | 0.009 | |
Vertical cortical index, % | 32.1a | 39.7b | 42.1b | 40.4b | 1.3 | <0.001 | |
Vertical wall thickness, mm | 1.01a | 1.61b | 1.92c | 1.67bc | 0.09 | <0.001 | |
Midshaft volume, cm3 | 1.17a | 1.71b | 2.22c | 1.69b | 0.12 | <0.001 | |
Moment of inertia, mm4 | 86.4a | 214.2b | 358.3c | 256.7b | 30.6 | <0.001 | |
Index of gyration, mm | 1.81a | 2.38b | 2.63c | 2.38b | 0.09 | <0.001 | |
Bone mechanical properties | |||||||
Yield strength, N | 163a | 357c | 293b | 197a | 21 | <0.001 | |
Ultimate strength, N | 240a | 441b | 464b | 289a | 25 | <0.001 | |
Elastic stress, MPa | 68.7b | 75.6b | 44.3a | 33.6a | 4.8 | <0.001 | |
Ultimate stress, MPa | 100.9b | 93.5b | 71.0a | 51.1a | 6.0 | 0.001 | |
Bending moment, N·m | 1.81a | 3.99c | 3.27b | 1.97a | 0.25 | <0.001 |
a, b, c–mean values in rows with different letters differ significantly at P<0.05; Data given are LSMeans; SEM–standard error of the mean
The description of the groups as in Table 1.