Fig 4. Effect of L-arginine supplementation on parasitological parameters and plasma urea cycle metabolites of mice infected with T. cruzi.

BALB/c mice were infected with T. cruzi and treated (or not) with L-arginine in the drinking water. L-arginine, ADMA and nitrites were quantified in plasma as described in the Materials and Methods. (A) Plasma L-arginine concentration at different d.p.i. (B) Plasma ADMA concentration at different d.p.i. (C) Plasma nitrite concentration (mean of 0, 14 and 21 d.p.i.). Mean ± SEM are shown from a representative experiment out of at least 3 independent experiments; n = 6 mice per experimental group, except for (C) where 3 mice were analyzed at each time point; *p<0.05; **p<0.005; ***p<0.001.