Fig 5. Effect of specific iNOS inhibitor 1400W in mice infected with T. cruzi with or without L-arginine supplementation.

BALB/c mice were infected with T. cruzi and supplemented with L-arginine or not in the drinking water, and treated or not with 1400W by IP inoculation. Parasitemia, survival and clinical score were monitored and nitrites were determined in plasma by the Griess method. (A) Parasitemia was determined by the Brener method. (B) Mean parasitemia of 9, 11, 13 15, 17 and 19 d.p.i. (C) Mean clinical scores of 18, 19 and 21 d.p.i. (D) Plasma nitrite concentration at 21 d.p.i. Mean ± SEM are shown from an experiment (n = 6 mice per experimental group, except for (D) where 3 mice per time point were analyzed); *p<0.05; **p<0.005; ***p<0.001).