Fig 2. HIV-1 BaL replication in MDMs treated with mifepristone and tamoxifen.

MDMs (1 x10 ⁶ cells/well) were pre-treated with concentrations of 5 μM tamoxifen or 0.6 nM mifepristone for 2 hours and appropriate concentrations of estrogen or progesterone were added and cultured for 5 days. The cells were infected with HIV-1 BaL at 5 ng/ml and cultured in media containing 110 nM estrogen and 64 nM progesterone. Culture supernatants were harvested 9 days post infection and HIV-1 replication quantitated by p24 ELISA. Culture supernatants were analyzed in triplicate. Results expressed as mean ± SEM are representative of three independent experiments.A. Tamoxifen and estrogen pre-treated MDMs B. Mifepristone and progesterone pre-treated MDMs.