Skip to main content
. 2018 Jan 16;14(1):e1007176. doi: 10.1371/journal.pgen.1007176

Fig 2. Tup1 binds along the WOR1 promoter differentially in white and opaque phases.

Fig 2

(A) ChIP of Tup1-HA in white and opaque cells. Overnight cultures of white and opaque cells of a wild-type strain (JYC5) and a strain carrying Tup1-HA (HLY4538) were diluted in SCD and grown to log phase at room temperature before formaldehyde. Enrichment is presented as a ratio of qPCR of the WOR1 promoter IP (bound/input) over an ADE2 control region IP (bound/input) of the tagged strain, further normalized to the control strain. Values are the average of three independent ChIP experiments with error bars representing the s.d. (B) Additional qPCR of Tup1 binding around -2.4kb upstream of the WOR1 TSS in white cells from (A).