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. 2018 Jan 16;14(1):e1007176. doi: 10.1371/journal.pgen.1007176

Fig 4. Tup1 depletion bypasses the requirement for Wor1 in the expression of WOR1 and key opaque regulators.

Fig 4

(A) Expression levels in the conditional Tup1 mutant and wor1 pMET3-tup1 conditional double mutant haploid strains. pMET3-TUP1 (HLY4533) and pMET3-TUP1 wor1 (HLY4539) were grown at room temperature for 48hr in the presence or absence of methionine. (B) Expression levels in diploid wor1 and wor1 tup1 mutant strains. Overnight cultures of MTLa/a wor1 (HLY3570), wor1 tup1 (HLY4540), and a control strain (JYC1) were inoculated into fresh SCD and grown to log phase. Expression levels of the indicated genes in (A) and (B) were measured by qPCR and normalized to ACT1. WOR1 expression was measured by qPCR using primers specific to either the WOR1 5’ UTR or the WOR1 coding region (CDS). Average expression level of three independent qPCR experiments was plotted with error bars representing the s.d.(C) Genetic model of Wor1-Tup1 regulation of WOR1 expression.