FIGURE 2.

Immunoblot analysis of the anti-N-terminus of α-enolase (NAE) antibodies. Anti- NAE antibodies (*) were detected in serum obtained from the patient 8 months after symptom onset. MW: the molecular weight marker; A: the serum obtained from Hashimoto encephalitis with anti- NAE antibodies as a positive control; B: the serum obtained from the present case 8 months after the onset; C: the serum obtained from the anti-NAE antibodies negative patient as a negative control. The bands at 97 kDa or above indicate immunological reactivity with derivatives from HEK293 cells, which were used to generate recombinant NAE protein. Bands greater than 97 kDa correspond to non-specific reactions with sera and appeared variously across samples. These bands were confirmed to be non-immunologically reactive with anti-His antibody, which could be used to verify expression of the recombinant NAE protein.^2,3