FIGURE 3.
Isolation and immunohistochemical detection of PrPD from RAMALT sample. (A) Dissection of recto-anal mucosal (RA) junction to isolate mucosa-associated lymphoid tissue (MALT) for RAMALT sample. (B) Immunohistochemistry of RAMALT from elk (Cervus elaphus nelsoni). Note the proteinase-resistant prion staining (red chromogen) distribution within the lymphoid follicle located in the lamina propria. Staining was done using a mouse monoclonal antibody F99/97.6.1 against epitopes at residues 220–225 of prion protein and developed with streptavidin-alkaline phosphatase method. The slides were further counterstained with hematoxylin (blue stain).