Table 1.

Primers sequences and MSP analysis conditions for miR-9-1, miR-9-3 and MTHFR genes.^24,25

	Primer (5'-3') F/R	Fragment Size (bp)	Anneling Temperature (°C)	Cycles
		110	55 (40 sec)	35
miR-9-1	tataagggtttcgtttcgtttttaac (M)
	acccgtcaccgaaaaaaa (M)
		110	60 (40 sec)	35
	ggttttgttttgtttttaatgt (U)
	caaccaaaaccctaccttcaac (U)
		116	58 (1 min)	35
miR-9-3	attggtcgatttttggattgac (M)
	cgcttaaaaaacctcgaacg (M)
		116	55 (1 min)	35
	gattggttgatttttggattgat (U)
	caaaacacttaaaaaacctcaaaca (U)
		180	58 (40 sec)	40
MTHFR	tagatttaggtaggtgaagtagggtagac(M)
	gaaaaactaataaaaaacggacgaa(M)
		180	58 (40 sec)	40
	tttaggtatgtgaaatagggtagatgt (U)
	caaaaaactaataaaaaaccaacaaa (U)

* F-Forward, R-Reverse, M-methylated, U-unmethylated, MSP- Methylation Specific PCR.