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Proline inhibits the GSAL site of the GSALDH module. (A) Electron density and interactions for proline bound in the GSAL site. The mesh represents a simulated annealing Fo–Fc omit map contoured at 3.0σ. (B) The active site of mouse GSALDH (ALDH4A1) complexed with the product glutamate (PDB ID 3V9K). Water molecules that mediate enzyme-ligand hydrogen bonds are represented by red spheres.
