Figure 2.

Mast cell accumulation in the peritoneum of mice induced by chymase and α₁‐chymotrypsin. (A), (B) and (C) Representative graphs of mast cell accumulation following injection of normal saline (NS), chymase (5 μg) and heat‐inactived chymase (hChymase, 5 μg) respectively. (D) Various concentrations of chymase (μg), mouse neutrophil elastase (μg) and HSA (μg) were injected i.p. in mice for 10 min and 3 h. (E) Various concentrations of HSA, elastase and chymase in the presence or absence of tryptase (Tryp, 0.5 μg), or α₁‐chymotrypsin (Chymot, μg) in the presence or absence of α₁‐antichymotrypsin (Antichym, 10 μg) were injected i.p. for 6 h. (F) Various concentrations of HSA, elastase and chymase in the presence or absence of Tryp, or Chymot in the presence or absence of Antichym were injected i.p. for 16 h. (G) Chymase (μg) and CI (μM) were injected i.p. in wild‐type C57BL/6J mice. Data are displayed as a boxplot, which indicates the median, interquartile range, the largest and smallest values. Each data group represents results from six to seven animals. *P < 0.05 compared with the corresponding NS group. ^† P < 0.05 compared with the corresponding stimulus alone group. ^‡ P < 0.05 compared with the corresponding chymase alone group.