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Inhibition of proteasomal degradation by αSyn A53T prolongs the biological half‐life of RTP801. (A) Cells transfected with pcDNA‐RTP801 were treated with CHX (10 μg·mL−1) for the indicated periods and lysed to examine the content of RTP801. (B) Cells transfected with pcDNA‐RTP801 were treated with or without MG‐132 (10 mM) for 24 h and subjected to Western blotting for RTP801. Data shown are means ± SEM, n = 5. *P<0.05, significantly different as indicated.
