. Author manuscript; available in PMC: 2019 Jan 25.

Published in final edited form as: Cell. 2018 Jan 4;172(3):618–628.e13. doi: 10.1016/j.cell.2017.12.009

KEY RESOURCES TABLE.

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies





Bacterial and Virus Strains
Escherichia coli W3110 Wild type, F⁻, λ⁻	Trent et al., 2001	N/A
Escherichia coli W3110, pmrA^C, polymyxin^R	Trent et al., 2001	N/A
Escherichia coli WD101ΔarnT:Cm^R	Herrera et al., 2010	ST01
Escherichia coli WD101ΔeptA:Cm^R	Herrera et al., 2010	ST02
Acinetobacter baumannii strain 17978	ATCC	ATCC 17978
Acinetobacter baumannii strain 5075	Jacobs et al., 2014	N/A
Pseudomonas aeruginosa strain PA14	Liberati et al., 2006	N/A
E. coli BAA-2452	ATCC	ATCC BAA-2452
E. coli W3110 carrying pSD03	This study	SD01
E. coli W3110 carrying pSD04	This study	SD02
E. coli WD101 carrying pSD05	This study	SD03
E. coli W3110 carrying pSD05	This study	SD04
WD101ΔarnT:Cm^R carrying pSD05	This study	SD05
WD101ΔeptA:Cm^R carrying pSD05	This study	SD06
E. coli W3110 carrying pSD06	This study	SD07
E. coli W3110 carrying pSD07	This study	SD08
A. baumannii 17978 carrying pSD07	This study	SD09
P. aeruginosa PA14 carrying pSD07	This study	SD10
E. coli W3110 carrying pSD08	This study	SD11
E. coli W3110 carrying pSD09	This study	SD12
E. coli W3110 carrying pMMB67EH	This study	SD13
E. coli W3110 carrying pSD10	This study	SD14
E. coli W3110 carrying pSD11	This study	SD15
E. coli C2987 chemically competent cells	NEB	Cat# C2987I


Biological Samples





Chemicals, Peptides, and Recombinant Proteins
Cecropin P1	Sigma	Cat# C7927
Denfensin HNP-1	Sigma	Cat# D2043
Protegrin	Anaspec	Cat# AS-64819-05
Peptide P1	Genscript	N/A
Peptide P2	Genscript	N/A
Peptide P3 cyclic^c	Genscript	N/A
Peptide P4	Genscript	N/A
Peptide P5 cyclic^d	Genscript	N/A
Peptide P6	Genscript	N/A
Peptide P7	Genscript	N/A
Peptide P8	Genscript	N/A
Peptide P9	Genscript	N/A
Peptide P10	Genscript	N/A
Peptide P11	Genscript	N/A
Peptide P12	Genscript	N/A
Peptide P13	Genscript	N/A
Peptide P14	Genscript	N/A
Peptide P15	Genscript	N/A
Peptide P16	Genscript	N/A
Peptide P17	Genscript	N/A
Peptide P18	Genscript	N/A
Peptide P3	Genscript	N/A
Peptide P5	Genscript	N/A
Control Peptide C1	Genscript	N/A

Critical Commercial Assays





Deposited Data
Raw and analyzed sequencing data	This study	GEO: GSE94531




Experimental Models: Cell Lines





Experimental Models: Organisms/Strains
Mouse: Envigo product 030 ICR (CD-1) outbred mice	Charles River Laboratories	Strain code: 022




Oligonucleotides
Peptide library oligonucleotides	IDT	Table S4

Recombinant DNA
pMMB67EH amp^R	ATCC	pMMB67EH
pMMB67EH with lpp ompA	This study	N/A
pMMB67EH with lpp ompA 2x tether	This study	N/A
pMMB67EH with lpp ompA cecropin P1	This study	N/A
pMMB67EH with lpp ompA 1x tether cecropin P1	This study	N/A
pMMB67EH with lpp ompA 2x tether cecropin P1	This study	N/A
pMMB67EH with lpp ompA 2x tether 2x HA	This study	N/A
pMMB67EH with lpp ompA 2x tether protegrin 1	This study	N/A
pMMB67EH with lpp ompA 2x tether defensin HNP-1	This study	N/A
pMMB67EH with lpp ompA 2x tether defensin HNP-1ΔC	This study	N/A
pMMB67EH with 2x tether cecropin P1	This study	N/A
pMMB67EH with lpp ompA 2x tether dermaseptin	This study	N/A

Software and Algorithms
GraphPad Prism 5 for Mac OS X	GraphPad Software, Inc	https://www.graphpad.com/scientificsoftware/prism, RRID:SCR_002798
Flexbar	Dodt et al., 2012	http://sourceforge.net/projects/flexbar, RRID:SCR_013001
Ustacks	Catchen et al., 2013; Catchen et al., 2011	N/A
Biopython	Cock et al., 2009	http://biopython.org, RRID:SCR_007173
DESeq2	Love et al., 2014	http://bioconductor.org/packages/release/bioc/html/DESeq.html, RRID:SCR_000154
R/Bioconductor	Huber et al., 2015	https://www.bioconductor.org, RRID:SCR_006442
FlowJo	Treestar, USA	http://www.flowjo.com, RRID:SCR_008520

Other