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. Author manuscript; available in PMC: 2019 Jan 25.
Published in final edited form as: Cell. 2017 Dec 21;172(3):549–563.e16. doi: 10.1016/j.cell.2017.11.043

Figure 5.

Figure 5

Four TIL-derived TCRs enrich the HLA-A*02:01 library for peptides.

(A) TCR sequences of the four orphan TCRs that selected peptides from the HLA-A*02:01 library. The TCR gene segments variable and joining are shown along with the corresponding CDR3 sequence. The abundance represents the amount of times a single cell was found to have the exact TCR sequence in tumor/healthy tissue.

(B) Nucleotide sequences of the two sequence-similar TCRs isolated from patients A and B. Non-encoded nucleotides are highlighted in red.

(C) HLA enrichment and tetramer staining per round of selection by the four orphan TCRs as measured by flow cytometry. The left panels indicate tetramer and epitope staining after all 4 rounds of selection have completed and the right panels indicate epitope staining through the course of selections.