Figure 2.

No significant effect on endothelial cell (EC) survival or permeability with microRNA (miR)-146a inhibition in vitro. (A,B) Twenty-four hours after transfection with 30 nM scramble or anti-miR-146a oligonucleotides, human umbilical vein endothelial cells (HUVECs) were exposed to 24 h of either growth [normoxia, 10% fetal bovine serum (FBS)] or hypoxia serum starvation (HSS) conditions. During the final 4 h, the tetrazolium salt WST-1 was added to the medium. Resulting salt cleavage to formazan by mitochondrial dehydrogenases was used as an indicator of cell viability (n = 4). Student’s t-test. (C) Transwell inserts (0.4 µm) were used to assess EC permeability via rhodamine dextran (70 kDa), after 24 h of HSS exposure in HUVECs transfected with scramble or anti-miR-146a oligonucleotides (n = 3). Mann–Whitney U-test. Data are mean ± SEM.