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. 2018 Jan 22;6:1. doi: 10.3389/fbioe.2018.00001

Figure 3.

Figure 3

Systemic delivery of anti-microRNA (miR)-146a-5p with single intramuscular injection. (A) Relative miR-146a-5p expression in the gracilis and gastrocnemius muscles of ligated limbs as well as the liver, spleen, and kidney 7 days post-femoral arterial ligation (FAL) in Balb/c mice. Mice were treated with a single i.m. injection of 7.5 nmol scramble (n = 3) or anti-miR-146a (n = 4) locked nucleic acid oligonucleotides immediately post-FAL. *p < 0.05, Student’s t-test except kidney samples which were Mann–Whitney U-test. (B) Sequence of miR-146a-5p and two additional miRNAs with similar seed sequences, miR-539-5p and miR-219c-3p. Seed sequence is highlighted in gray with nucleotides altered from miR-146a-5p sequence underlined. (C) Expression of miR-539-5p and miR-219c-3p in gastrocnemius of ligated limb 7 days post-FAL (n = 3–4 for scramble and anti-miR-146a treated mice, respectively). Student’s t-test. Data are mean ± SEM.