Table 2.

Characteristics of ANA-reactive mAbs derived from graft-versus-host disease (GVHD) mice.^a,b

Mouse number	Hybridoma number	IgG isotype	Ig-heavy chain v-region			Ig-light chain v-region
GVHD # 1	1.12.1A	IgG2b	Vh8-8	D2-14	J4	Vk4-74	Jk2
	1.28.1	IgG2a	Vh1-26	D1-1	J1	Vk4-74	Jk2
	1.308.1	IgG2a	Vh1-55	D2-4	J3	Vk3-4	Jk1
	1.356.1	IgG2a	Vh1-55	D2-4	J3	Vk3-4	Jk1
	1.741.1	IgG2a	Vh1-55	D2-4	J3	Vk3-4	Jk1
	1.802.1	IgG2b	Vh1-26	D1-1	J1	Vk4-63	Jk5
GVHD # 2	2.24.0	IgG2a	Vh1-52	D1-1	J1	Vk14-111	Jk5
	2.53.8	IgG2b	Vh14-2	D2-2	J2	Vk14-111	Jk1
	2.58.2	IgG2b	Vh14-2	D2-2	J2	Vk14-111	Jk1
	2.70.8	IgG2a	Vh14-4	D1-1	J3	Vk3-10	Jk1
	2.80.9	IgG2a	Vh14-4	D1-1	J3	Vk3-12	Jk1
	2.152.9	IgG2a	Vh1-52	D1-1	J1	Vk3-10	Jk1
	2.300.2	IgG2b	Vh1-55	D2-1	J2	Vk3-10	Jk5
	2.344.3	IgG2a	Vh1-52	D1-1	J1	Vk14-111	Jk5
	2.374.1	IgG2a	Vh1-26	D1-1	J1	Vk3-7	Jk1
	2.382.2	IgG2a	Vh14-4	D2-1	J3	Vk3-10	Jk1
	2.388.2	IgG2a	Vh1-31	D2-4	J4	Vk3-10	Jk2
	2.394.1	IgG2a	Vh14-4	D1-1	J3	Vk3-10	Jk1
GVHD # 3	3.55.1	IgG2a	Vh1-26	D1-1	J3	Vk4-74	Jk2
	3.69.1	IgG2b	Vh1-26	D1-1	J3	Vk4-74	Jk2
	3.71.1	IgG2a	Vh1-26	D2-5	J1	Vk4-74	Jk2
	3.77.1	IgG2a	Vh1-26	D1-1	J3	Vk4-74	Jk2
	3.152.1	IgG2b	Vh1-26	D1-1	J3	Vk4-74	Jk2
GVHD # 4	63.3.1	IgG2b	Vh1-55	D2-4	J2	Vk4-74	Jk2
	165.2.1	IgG2a	Vh8-12	D2-2	J1	Vk15-103	Jk5
GVHD # 5	14.11.A	IgG2b	Vh1-50	D1-1	J2	Vk1-117	Jk2
	381.11.A	IgG2b	Vh1-54		J4	Vk14-111	Jk2
	685.9.A	IgG2b	Vh1-59	D2-5	J3	Vk17-127	Jk5
	724.3.A	IgG1	Vh1-26	D1-1	J3	Vk4-91	Jk4
	738.8.A	IgG1	Vh1-26	D1-1	J3	Vk3-7	Jk1
	762.5.A	IgG2a	Vh5-17	D1-2	J4	Vk?
	810.4.A	IgG1	Vh1-52	D2-12	J2	Vk3-10	Jk1
	865.12.A	IgG2b	Vh1-72	D3-3	J2	Vk4-74	Jk5
	1011.39.A	IgG2b	Vh1-53	D1-1	J2	Vk17-127	Jk5

^aAll the mAbs converted into germline sequences are boxed in orange.

^bAll the mAbs using Vh1-26 are boxed in yellow and those using Vk4-74 in red.