Table 3.

Vh-CDR2 regions of non-Vh1-26 ANA-reactive mAbs of graft-versus-host disease (GVHD) and aging B6 mice.^a,b

Mouse number	Hybridoma number	Vh-region	Vh-CDR2 region	Mouse number	Hybridoma number	Vh-region	Vh-CDR2 region
GVHD # 1	1.12.1A	Vh8-8	I W W D D D	Aging B6 # 1	7.F9.F1	Vh1-75	I L P G S G S S
	1.308.1	Vh1-55	I Y P G S G S T
	1.356.1	Vh1-55	I Y P G S G S T	Aging B6 # 2	1.G5.B8	Vh1-39	V N P N Y G T I
	1.741.1	Vh1-55	I Y P G S G S T
				Aging B6 # 6	3.2.2A	Vh1-74	I H P S D S D T
GVHD # 2	2.24.0	Vh1-52	I D P S D G E T	Aging B6 # 6	6.15.1A	Vh8-12	I Y W D D D K
	2.53.8	Vh14-2	I D P E D G E T		7.7.3A	Vh1-22	I N P N N G G T
	2.58.2	Vh14-2	I D P E D G E S		20.15.1A	Vh1-50	I D P S D T F T
	2.70.8	Vh14-4	I D P E N G D T		23.6.1A	Vh1-22	I N P N N G D T
	2.80.9	Vh14-4	I D P E N G D T		24.18.1A	Vh1-22	I N P N N D D T
	2.152.9	Vh1-52	I D P S D G E T		25.8.1A	Vh3-6	I S C D G S S
	2.300.2	Vh1-55	I Y P G S V S T
	2.344.3	Vh1-52	I D P S D D E T	Aging B6 # 7	3.25A	Vh1-50	I D P S D T Y T
	2.382.2	Vh14-4	I D P E N G D T	Aging B6 # 7	3.36A	Vh1-80	I Y P G D G D T
	2.388.2	Vh1-31	I F P Y N G V S
	2.394.1	Vh14-4	I D P E N G D T
GVHD # 4	63.3.1	Vh1-55	I Y P G S G S T
	165.2.1	Vh8-12	I Y W D D D K
GVHD # 5	14.11.A	Vh1-50	I D P S D S Y I
	381.11.A	Vh1-54	I N P G S G G I
	685.9.A	Vh1-59	I D P S D S S S
	762.5.A	Vh5-17	I S R G S G I L
	810.4.A	Vh1-52	I D P S D S E T
	865.12.A	Vh1-72	I D P S S G G T
	1011.39.A	Vh1-53	I N P S N D G T
	1011.39.A	Vh1-53	I N P S N D G T

Bold font highlights negatively and positively charged amino acids.

^aAll mAbs that were converted into germline sequences or in which the negatively charged amino acids in the CDR2 were converted into glycine are marked with orange boxes.

^bAll the negatively charged amino acids in Vh-CDR2 region are marked in yellow and the positively charged amino acids in green.