Figure 3.

The homozygous Taar1^m1J genotype is associated with higher MA intake. (A) Mean ± SEM MA consumption when MA was offered vs. water in 20 and 40 mg/l concentrations for F2 mice selected to produce the S1 generation MADR line offspring of replicate (Rep) 3–5, separated by Taar1 genotype; Taar1 genotype frequencies for these mice are those in Figures 2A,B. (B) Mean ± SEM total amount of fluid consumed by the same F2 mice from both the water and MA tubes, during the 18-h period when MA was available. (C) Mean ± SEM MA consumption when MA was offered vs. water in 20 and 40 mg/l concentrations to MADR mice of several replicates and selection generations, for which Taar1 genotype frequencies are shown in Figure 2C. (D) Mean ± SEM total amount of fluid consumed by the same MADR mice from both the water and MA tubes, during the 18-h period when MA was available. ^***p < 0.001 for the comparison of homozygous Taar1^m1J to heterozygous and homozygous Taar1⁺ mice; +++p < 0.001 for the comparison of MA consumption from the 20 and 40 mg/l MA concentrations within the homozygous Taar1^m1J mice; ^#p < 0.05 for overall more MA consumed in Rep 5 compared to Rep 3 and Rep 4 homozygous Taar1^m1J mice; ###p < 0.001 for overall more MA consumed in Rep 3 S3 Taar1^m1J mice, compared to Rep 4 S1 and Rep 5 S1 and S2 Taar1^m1J mice.