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. 2017 Dec 18;13(2):54–61. doi: 10.46582/jsrm.1302009

Figure 3.

Figure 3.

Effects of neural stem/progenitor cells (NSPCs) grown with or without fetal calf serum (FCS) upon maturation of dendritic cells (DCs). NSPCs were co-cultivated with immature DCs at 1:1 and 1:5 NSPCs/DCs ratios in the presence of bacterial lipopolysaccharide (LPS). After 3 days in co-culture the cells were suspended, double stained with fluorescent monoclonal antibodies (MoAbs) against CD83 and HLA-DR and analyzed by flow cytometry. (A, B) Combined data for 3 “C” type (serum-free medium) and 4 “S” type (FCS-containing medium) NSPCs cultures. (A) Percentage of DCs expressing CD83. (B) Median of fluorescence intensity of MoAbs against HLA-DR. (C, D) Histograms showing CD 83 (C) and HLA-DR (D) expression level for DCs co-cultured with S163 (representative experiment). Black curves — C163 co-culture; black broken curves — MSCs co-culture; grey broken curves — DCs monoculture without LPS. The percentages of cells within the defined populations given on top.