FIG 5.

Effects of Ψ loss on stop codon readthrough, +1 and −1 frameshifting, initiation on non-AUG codons and leaderless mRNA, and T4 g60 bypassing in lacZ reporter constructs. Assay results are presented as Miller units of β-galactosidase activity. Each bar represents the mean of measurements from at least three independent cultures ± SD. An engineered, wild-type lacZ plasmid, pSG25, supported 22,246 ± 884 and 27,318 ± 2,353 Miller units of activity in the wild-type and ΔΨ7 strains, respectively.