TABLE 2.

Case study of clinical CSF specimens that were culture negative and tested positive by multiplex LightMix RT-PCR

Case study	Sample	Multiplex RT-PCR result	Microscopic examination result	Clinical feature(s)
70-year-old female	CSF	S. pneumoniae	Cytospin preparations showed mononuclear and polymorphonuclear leukocytes (cell count of >103/ml)	Clinical suspicion of pneumococcal sepsis
			No bacteria were detected by microscopic examination	Patient under antibiotic treatment
39-year-old male	CSF	S. pneumoniae	Cytospin preparations showed mononuclear and polymorphonuclear leukocytes (cell count of >103/ml)	Clinical suspicion of pneumococcal meningitis
			No bacteria were detected by microscopic examination	Patient under antibiotic treatment
58-year-old male	CSF	S. agalactiae	Cytospin preparations showed mononuclear and polymorphonuclear leukocytes (cell count of >103/ml)	Clinical suspicion of meningoencephalitis
			No bacteria were detected by microscopic examination	Patient under antibiotic treatment
26-year-old male	CSF	N. meningitidis	Cytospin preparations showed mononuclear and polymorphonuclear leukocytes (cell count of >103/ml)	Clinical suspicion of meningococcal meningitis
			Gram-negative diplococci were detected by microscopic examination	Patient under antibiotic treatment