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Schematic view of the sigE gene fragment targeted by the PCR-RFLP assay. Arrows show primers. The asterisk shows the AluI site created in the case of the codon 98 CTG→CTA mutation in a Beijing 94-32 cluster isolate. Gray boxes show particular codons. The permanent AluI site (codons 86 to 87) was observed in all genomes in the GMTV database and thus does not interfere with PCR-RFLP analysis of codon 98. F, forward; R, reverse; WT, wild type.
