TABLE 1.
Tool | No. (%) available |
|||||
---|---|---|---|---|---|---|
For HLIU | Within patient care room | Within isolation unit | Within facility | Outside facilitya | Other | |
Incubator for bacterial cultureb | 30 (94) | 0 (0) | 8 (27) | 19 (63) | 2 (7) | 1c (3) |
Biological safety cabinet | 31 (97) | 0 (0) | 17 (55) | 14 (45) | 0 (0) | 0 (0) |
PCR assay | 28 (88) | 0 (0) | 9d (32) | 11d (39) | 8e (29) | 0 (0) |
EIA readerf | 19 (59) | 1 (5) | 4 (21) | 11 (58) | 2 (11) | 1c (5) |
Microscope | 23 (72) | 0 (0) | 6 (26) | 17 (74) | 0 (0) | 0 (0) |
Including the jurisdictional PHL (excluding the CDC laboratory for confirmation diagnosis).
Including the availability of a standalone incubator for bacterial culture; although this did not include automated blood culture systems, many facilities preferred to incubate blood culture bottles in a standalone incubator for visual observation, with Gram staining and culture performed when necessary.
The health system's core laboratory.
PCR testing within the isolation unit or facility generally included access to BioFire instrumentation (BioFire, Salt Lake City, UT), including the FDA emergency use authorization-approved FilmArray Biothreat Etest to test for the presumptive presence of Ebola Zaire virus, as well as FDA-approved FilmArray assays including panels for blood culture identification (BCID) and gastrointestinal tract and respiratory tract pathogens.
Jurisdictional PHLs utilized real-time PCR assays developed by the CDC and validated in-house to test for pathogens such as Ebola Zaire virus, novel Middle Eastern respiratory syndrome (MERS) coronavirus, and influenza A/H7 virus.
Including enzyme immunoassay (EIA) readers for the direct detection of agents such as influenza viruses, group A Streptococcus, HIV, and malaria.