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. 2018 Jan 25;62(2):e01872-17. doi: 10.1128/AAC.01872-17

FIG 3.

FIG 3

F-Hst 5 is taken up by C. auris cells. F-Hst 5 (30 μM) and PI (0.5 μM) were added to C. auris cells adherent to a glass coverslip, and intracellular uptake was imaged after 30 min at 23°C using DIC and fluorescence confocal microscopy. Regardless of the killing efficiency of Hst 5, both CAU-07 and CAU-08 transported F-Hst 5 to the cytosol (as visualized by green florescence), resulting in a change in cell topology followed by cell death (as visualized by red PI staining). Bar, 5 μm.