TABLE 1.

Phenotypic characterization of C. auris isolates used in this study

Strain no.	C. auris strain designation	Cell shape	Aggregationa	Hyphae or pseudohyphaeb	Colony morphology on Spider medium	Color on CHROMagarc	Chlamydospore productiond
CAU-01	AR-BANK#0381	Ellipsoidal	Single cells	None	Smooth colonies	Pink	None
CAU-02	AR-BANK#0382	Ellipsoidal	Aggregative	None	Smooth colonies	Pink	None
CAU-03	AR-BANK#0383	Spherical to ovoid	Aggregative	None	Smooth colonies	Dark pink	None
CAU-04	AR-BANK#0384	Spherical to ovoid	Aggregative	None	Smooth colonies	Dark pink	None
CAU-05	AR-BANK#0385	Spherical to ovoid	Aggregative	None	Smooth colonies	Dark pink	None
CAU-06	AR-BANK#0386	Spherical to ovoid	Aggregative	None	Smooth colonies	Dark pink	None
CAU-07	AR-BANK#0387	Ellipsoidal	Single cells	None	Smooth colonies	Pink	None
CAU-08	AR-BANK#0388	Spherical to ovoid	Single cells	None	Smooth colonies	Pink	None
CAU-09	AR-BANK#0389	Spherical to ovoid	Single cells	None	Smooth colonies	Pink	None
CAU-10	AR-BANK#0390	Ellipsoidal	Single cells	None	Smooth colonies	Dark pink	None

^aCells were mixed in PBS and were vortexed at high speed for 1 min before microscopy to detect cell aggregation.

^bHyphal formation was assessed in BD Difco yeast extract-peptone-dextrose (YPD) broth, yeast nitrogen base (YNB) with ammonium sulfate supplemented with 1.25% N-acetyl-d-glucosamine, YPD broth with 10% fetal bovine serum, and Spider medium. Each medium was inoculated with 1 × 10⁷ cells/ml, and cells were incubated at 37°C with agitation for as long as 24 h.

^cC. auris strains were streak-inoculated in CHROMagar Candida medium and were incubated at 37°C for 48 h in order to observe colony morphology.

^dC. auris strains were inoculated onto corn meal agar and rice extract agar using the Dalmau technique, incubated at 23°C in the dark for as long as 2 weeks, and observed microscopically for the presence of chlamydospores.