FIG 9.

RT-qPCR analysis of Prnp mRNA expression in N2a cells. Cells were treated with the test compounds (J1, J8, J20, and Y17) at 10 μM or the vehicle (DMSO 0.1%). Prnp mRNA levels were normalized to the Gapd mRNA content and were expressed relative to the value for untreated cells (UT). One-way ANOVA was performed using GraphPad Prism software. Error bars indicate standard errors of the means (SEM). The number of independent experiments for each condition was 4 for untreated cells, 1 for vehicle-treated cells, 1 for J1-treated cells, 3 for J8-treated cells, 2 for J20-treated cells, and 1 for Y17-treated cells.