TABLE 2.
Activity of IQG-607, INH, ETH, and MFX against several laboratory strains of M. tuberculosis
| Strain | katG genotype | inhA promoter genotype | inhA genotype | MIC (mg/liter) fora |
|||
|---|---|---|---|---|---|---|---|
| INH | ETH | MFX | IQG-607 | ||||
| H37Rv | WT | WT | WT | 0.39 | 3.13 | 0.08 | 1.56 |
| IQG1b | 120del1 | WT | WT | >100 | 3.13 | 0.16 | >100 |
| IQG2 | 120del1 | WT | WT | >100 | 1.56 | 0.08 | >100 |
| IQG3 | T(271)C | WT | WT | >100 | 3.13 | 0.16 | >100 |
| IQG4 | 1298del1 | WT | WT | >100 | 1.56 | 0.08 | >100 |
| IQG5 | 120del1 | WT | WT | >100 | 1.56 | 0.02 | >100 |
| IQG6 | C(1249)G | WT | WT | >100 | 3.13 | 0.08 | >100 |
| pNIP::furA+katG(WT) | WT | WT | WT | <0.2 | 3.13 | <0.2 | 0.78 |
| pNIP::furA+katG(S315T) | G(944)Cc | WT | WT | >100 | 0.78 | <0.2 | >100 |
| pNIP::Ø | WT | WT | WT | 0.39 | 6.25 | <0.2 | 1.56 |
| pNIP::InhA(WT) | WT | WT | WT | 0.39 | 6.25 | <0.2 | 3.13 |
| pNIP::InhA(S94A) | WT | WT | T(280)Gd | 25 | >100 | <0.2 | >100 |
a
MIC values reported here were observed in two independent experiments or were the highest value found among three independent tests. ETH, ethionamide.
b
IQG1-6 refers to the IQG-607 spontaneous mutant strains.
c
This mutation changes the serine-315 residue by a threonine (S315T).
d
This strain still expresses the underlying level of the wild-type inhA gene but carries the mutant inhA gene [T(280)G], cloned into the integrative plasmid pNIP40/b. This mutation changes the serine-94 residue by an alanine (S94A).