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. Author manuscript; available in PMC: 2019 Jan 22.
Published in final edited form as: Curr Biol. 2017 Dec 28;28(2):196–210.e4. doi: 10.1016/j.cub.2017.11.069

Figure 2. The TORC2 network is modulated by nutrients.

Figure 2

(A) Wildtype and rts1Δ cells were grown to early log phase at 22°C in YPD medium. Cells were washed into YPG/E medium and the behavior of Mss4-3XHA was assayed by western blotting at the indicated times. (B) Wildtype and rts1Δ cells were shifted from rich to poor carbon as in (A). Ypk-pT662, Ypk-pT504 and Ypk1 were assayed by western blot. (C) Wildtype and rts1Δ cells were grown at 22°C to early log phase in YEP media containing glucose (Glu), galactose (Gal) or glycerol/ethanol (GE). Ypk-pT662, Ypk-pT504 and Ypk1 were assayed by Western blotting. An asterisk indicates a non-specific band. See also Figure S2 and Table S1.