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. Author manuscript; available in PMC: 2019 Jan 22.
Published in final edited form as: Curr Biol. 2017 Dec 28;28(2):196–210.e4. doi: 10.1016/j.cub.2017.11.069

Figure 7. Ceramides are required for nutrient modulation of the TORC2 signaling network.

Figure 7

(A–B) Wildtype and lac1Δ lag1Δ cells were grown to early log phase in YPD at 22°C. After washing into YPG/E, cells were incubated at 25°C and samples were taken at the indicated times. Mss4-3XHA was detected by western blot. Ypk-pT662, Ypk-pT504 and Ypk1 were assayed by western blot. An asterisk indicates a non-specific band. (C) Quantification of ceramides and complex sphingolipids in wild type and rts1Δ cells growing in rich or poor carbon. Data represent the average of three biological replicates. A Welch Two Sample t-test is used to estimate the p-values: * p < 0.05, ** p < 0.01. (D) A model for how nutrient-dependent modulation of the TORC2 signaling network could influence cell growth and size. See also Figure S7, Table S1 and Data S1.