Skip to main content
. 2018 Jan 23;8:2006. doi: 10.3389/fimmu.2017.02006

Figure 4.

Figure 4

Global gene expression analysis of identified genes closely related to positive selection, in which major histocompatibility complex (MHC) molecules and CD127 are downregulated in thymic stromal cells and thymocytes, respectively. (A) Forty-nine downregulated genes were classified into nine groups based on their functions, in which nine (18.3%) genes are closely related to the positive selection process. (B–E) Thymic stromal cells were isolated from gender- and age-matched wild-type (WT) and transgenic (Tg) mice and then stained with anti-CD45, CD11b, CD11c, and MHC class I/II antibodies. The median florescence intensity (MFI) of MHC class I or II was determined in the CD45−, CD45+CD11b+, and CD45+CD11c+ population using flow cytometry (FCM). (F–I) Thymocytes were isolated from gender- and age-matched WT and Tg mice, and the expression (indicated by MFI) of CD127 (F,G) and Bcl-2 (H,I) was analyzed using FCM. Each dot represents one mouse, and the values represent the mean ± SD. Statistical significance is indicated as follows: *P < 0.05, **P < 0.01, *** and P < 0.001 (Holm–Sidak method).