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. 2018 Jan 23;9:4. doi: 10.3389/fphar.2018.00004

FIGURE 5.

FIGURE 5

Effect of CP-25 on proliferation of human peripheral blood mononuclear cells (PBMCs) and CD4+ T cells stimulated with immunoglobulin D (IgD) in vitro. CD4+ T cells in each group were 98% viable, based on trypan blue staining. PBMCs were cultured with different concentrations of CP-25 (10-9∼10-5 mol/l) combined with IgD (3 μg/ml) for 24, 48, and 72 h, and the viability of PBMCs was detected by CCK-8 (A). Purified CD4+ T cells were cultured with IgD alone or CP-25 in addition. The viability and proliferation of T cells were separately detected by CCK-8 (B) and CFSE analysis (C). Data are expressed as the mean ± standard error of the mean (n = 3). ∗P < 0.05, ∗∗P < 0.01 vs. control; #P < 0.05, ##P < 0.01 vs. IgD (3 μg/ml) group.