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. 2018 Jan 23;9:4. doi: 10.3389/fphar.2018.00004

FIGURE 6.

FIGURE 6

Effect of CP-25 on the proportions of T cell subsets and the expression of immunoglobulin D receptor (IgDR) in human peripheral blood mononuclear cells (PBMCs) induced by immunoglobulin D (IgD). Human PBMCs were cultured with different concentrations of CP-25 (10-9∼10-5 mol/l) combined with IgD (3 μg/ml) for 24 h. Flow cytometry was used to analyze the effects of CP-25. After incubation, the expression of IgDR was detected using biotinylated IgD followed by APC-Cy7-conjugated streptavidin. Cells stained with APC-Cy7 conjugated streptavidin alone were used as an isotype control. (A) Representative flow cytometry dot plot from each group. Bar graph illustrating the proportion of CD4+/CD154+ (B), CD4+/CD69+ (C), CD4+/CD62L+ (D), CD3+/IgDR+ (E), CD3+/CD4+/IgDR+ (F), and CD4+/CD154+/IgDR+ (G) T cells. Data are expressed as the mean ± standard error of the mean (n = 5). ∗P < 0.05, ∗∗P < 0.01 vs. control group; #P < 0.05, ##P < 0.01 vs. IgD group.