Figure 2.

Confocal microphotograph of vertical limb of the diagonal band of Broca (HDB) showing labeled neurons of animals injected with the fluorescent tracers in the left hemisphere in S1 (yellow neurons) and A1 (blue neurons). Since the injection was in the left hemisphere, ipsilateral neurons are at the left side of the dash line (indicated above) and contralateral neurons are the ones located at the right side of the dash line indicating the middle line (upper images). Bottom left image shows positive neurons for choline-acetyltransferase (ChAT) immunostaining labeled in red color. FB: Fast Blue (blue) labeled neurons; Fluoro-Gold (FlGo; yellow): FlGo labeled neurons; ChAT (red): cholinergic labeled neurons. Labelled neurons were observed both in the ipsi (left hemisphere) and the contralateral (right) injection site.