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. 2018 Jan 23;12:5. doi: 10.3389/fnana.2018.00005

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Copyright © 2018 Chaves-Coira, Rodrigo-Angulo and Nuñez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal microphotographs of labeled neurons in horizontal limb of the diagonal band of Broca (HDB) nuclei of the basal forebrain (BF) of animal injected with both fluorescent tracers in the left hemisphere in S1 (yellow neurons) and A1 (blue neurons). The left column shows the HDB labeled neurons of the ipsilateral injection site; the right column shows the HDB contralateral labeled neurons to the injection site. Blue indicated FB labeled neurons; Yellow indicated FlGo labeled neurons and red color indicates positive neurons for ChAT immunostaining. The upper row shows the merge of all labeled neurons in HDB.