Fig. 1.

PD-1 and PD-L1 antibody blockade inhibit growth of CMT167 orthotopic lung tumors. CMT167 orthotopic tumor-bearing mice were injected with anti-PD-1, anti-PD-L1, or isotype control antibody (n = 5 for each group). Effects of anti-PD-1/anti-PD-L1 therapy on (A) CMT167 primary tumor volume and (B) metastatic burden in the other lung lobes. Each point represents an individual mouse. (C) Representative CD3 immunostaining (FITC, green) of sections from mice treated with control IgG2a (a and b), anti-PD-1 (c and d), control IgG2b (e and f), and anti-PD-L1 (g and h). Nuclei were stained with DAPI (blue); tumor borders are indicated by arrowheads. (a,c,e,g) Low power image (10x), scale bar = 200 μm; (b,d,f,h) high power detail of boxed regions from low power images (40x), scale bar = 100 μm.