Table 1.
Strains and plasmids used in this study
| Strains and plasmids | Relevant genotype or description | Source or references |
|---|---|---|
| Strains | ||
| B. subtilis | ||
| PKS11 | trpC2 pLS20cat | [15] |
| GR138 | trpC2 pLS20cat pGR16B | [19] |
| TMO310 | trpC2 aprE::(spc lacI Pspac-mazF) | [20] |
| TMO311 | trpC2 aprE::(kan lacI Pspac-mazF) | [20] |
| YNB001 | trpC2 comK::spc | This study |
| YNB022 | trpC2 pLS20cat (kan lacI Pspac-mazF) | This study |
| YNB026 | trpC2 pLS20catΔoriT | This study |
| YNB031 | trpC2 pLS20catΔoriT pGR16B | This study |
| YNB060 | trpc2 aprE::kan yhfM::(oriTLS20-F erm) | This study |
| YNB061 | trpc2 aprE::kan yhfM::(oriTLS20-R erm) | This study |
| YNB069 | trpc2 aprE::kan yhfK::(oriTLS20-F erm) | This study |
| YNB062 | trpc2 aprE::kan yhfC::(oriTLS20-F erm) | This study |
| YNB097 | trpc2 aprE::kan yhcT::(oriTLS20-F erm) | This study |
| YNB065 | trpc2 aprE::kan yhfM::(oriTLS20-F erm) pLS20cat | This study |
| YNB066 | trpc2 aprE::kan yhfM::(oriTLS20-R erm) pLS20cat | This study |
| YNB071 | trpc2 aprE::kan yhfK::(oriTLS20-F erm) pLS20cat | This study |
| YNB067 | trpc2 aprE::kan yhfC::(oriTLS20-F erm) pLS20cat | This study |
| YNB099 | trpc2 aprE::kan yhcT::(oriTLS20-F erm) pLS20cat | This study |
| YNB091 | trpc2 aprE::kan yhfM::(oriTLS20-F erm) pLS20catΔoriT | This study |
| YNB095 | trpc2 aprE::kan yhfM::(oriTLS20-R erm) pLS20catΔoriT | This study |
| YNB092 | trpc2 aprE::kan yhfK::(oriTLS20-F erm) pLS20catΔoriT | This study |
| YNB094 | trpc2 aprE::kan yhfC::(oriTLS20-F erm) pLS20catΔoriT | This study |
| YNB100 | trpc2 aprE::kan yhcT::(oriTLS20-F erm) pLS20catΔoriT | This study |
| Plasmids | ||
| pLS20cat | Conjugative plasmid pLS20 with a chloramphenicol resistance gene inserted in the unique Sall site | [18] |
| pLS20catΔoriT | pLS20cat without oriTLS20 | This study |
| pGR16B | Mobilizable plasmid containing oriTLS20 and erythromycin resistance gene | [19] |