Hyperglycemia suppressed the expression of matrix metalloproteinases, MMP1 (A) and MMP13 (B). T2DM influenced the expression of TIMP1 (C), TIMP2 (D), TIMP3 (E), and TIMP4 (F). Hyperglycemia activated collagen genes, COL1A2 (G) and COL4A (H). Each experiment was repeated three times. * indicates significant difference (P < 0.05) when compared with the ND group.