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. Author manuscript; available in PMC: 2018 Jan 28.
Published in final edited form as: J Am Coll Surg. 2008 Oct 10;208(1):110–119. doi: 10.1016/j.jamcollsurg.2008.08.012

Figure 4.

Figure 4

Cytokine levels in leukoreduction (LR) packed red blood cell (PRBC) supernatant decreases with storage and exposure of peripheral blood mononuclear cells to PRBC supernatant does not result in substantial cytokine alterations. (A) LR PRBC supernatant (n = 3) was analyzed by bead array or standard ELISA (for transforming growth factor [TGF]–β1) for cytokine concentrations. D42 LR PRBC supernatants had statistically significant decreases in all cytokines except for TGF-β. (B) Peripheral blood mononuclear cells were exposed to control media (Ctl) or PRBC supernatant from leukoreduced units after 1 day or 42 days of storage (D1 LR or D42 LR) ± anti-CD3 stimulation. The supernatant was collected and cytokines were measured. Compiled data from three independent experiments expressed as mean ± SEM. *p < 0.05 versus Ctl + anti-CD3 group.