Figure 5.
Washed PRBC supernatant does not induce regulatory T cells (Tregs) or activate T cells. Peripheral blood mononuclear cells (PRBC) were exposed to control media (Ctl) or washed packed red blood cell supernatant after 7 days or 42 days of storage ± anti-CD3 stimulation. Compiled data from three independent experiments expressed as mean % (A) Foxp3+ Tregs or (B) CD25+Foxp3− activated T cells of all CD4+ T cells ± SEM.