Extended Data Figure 8. Role of Dhx9 in Nlrp9b-mediated antirotavirus response.

a, Biotin-labelled low molecular weight poly(I:C) were incubated with whole-cell lysates from HEK293T cells transfected with Flag-NLRP9 and indicated siRNA. b, HEK293T cells were transfected with DHX9-targeting siRNA or control siRNA for 24 h, co-transfected with Flag-NLRP9 and HA-ASC for another 24h, and infected with rotavirus human rotavirus WA strain (estimated MOI = 2) for 6 h. Cell lysates were co-immunoprecipitated with anti-Flag antibody and analysed by western blot with the indicated antibodies. c, Validation of Dhx9 conditional knockout mice. Dhx9 mRNA expression in intestinal epithelial cells from indicated mouse strains (mean ± s.e.m., Student’s t-test, **P < 0.01, ***P < 0.001). d, Cytotoxicity measured by lactate dehydrogenase (LDH) release in organoid cells from the indicated mouse strains infected with rotavirus EW strain (5 × 10⁵ DD₅₀, estimated MOI = 5) for 3 h (mean ± s.e.m., Student’s t-test, *P < 0.05). e, ELISA measurement of Il-18 secretion upon treating intestinal organoid cells from the indicated mouse strains with rotavirus for 3 h (mean ± s.e.m., Student’s t-test, **P < 0.01). f, qPCR detection of viral loads by measuring virus transcript VP6 in organoid cells from the indicated mouse strains infected with rotavirus for 6 h (mean ± s.e.m., Student’s t-test, *P < 0.05).