a, Workflow to generate Flag–Nlrp9 -knock-in mice. b, Immunoprecipitation and western blots detection of Flag-Nlrp9b protein, c, qPCR analysis of Nlrp9b, Asc and rotavirus transcripts, in IECs samples from Flag-Nlrp9b-knock-in mice that were infected with 100 DD50 of rotavirus by the indicated time points. d, Expression of select innate immune receptors or adaptors were measured by qPCR in different intestine sections isolated from mice with different ages as indicated in the figure. (mean ± s.e.m., Student’s t-test compare to expression levels in day 0, *P < 0.05, **P < 0.01.) Experiments in b and c were replicated twice.