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. 2018 Jan 26;4(1):eaaq1407. doi: 10.1126/sciadv.aaq1407

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Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 4 — (A) SIM of the ALA-fed E. coli DE/BoP/IG cells. The color bar shows the color map of the image. The inset image shows the cellular distribution of Chl a in more detail. Scale bars, 5 μm. E. coli 3a cells with or without ALA feeding showed zero or limited fluorescence. (B to E) Bright-field and epifluorescence images of the E. coli 3a cells without (B and C) and with ALA feeding (D and E). Scale bars, 5 μm. (F and G) Fluorescence images of the ALA-fed E. coli (F) DE/BoP/IG and (G) 3a cells immobilized on an agar gel pad. Scale bars, 5 μm. (H) Fluorescence emission spectra recorded from individual E. coli DE/BoP/IG cells [marked with a front sight symbol in (G)]. a.u., arbitrary units.