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. 2017 Dec 19;9(1):1169–1186. doi: 10.18632/oncotarget.23453

Figure 6. Effects of estradiol on induction of mitochondrial cytochrome c oxidase (COX) I and II mRNA expressions.

Figure 6

Human osteoblast-like U2OS cells were exposed to 10 nM of estradiol for 1, 3, 6, 12, 18, and 24 h. Levels of COX I and II mRNA were analyzed using an RT-PCR (A and C, top panels). Amounts of β-actin mRNA were assayed as the internal standard (bottom panel). These bands were quantified and statistically analyzed (B and D). A quantitative real-time PCR analysis was carried out to confirm expression of COX I mRNA in U2OS cells and rat calvarial osteoblasts (E). Each value represents the mean ± SEM for n = 6. The symbol * indicates that the value significantly differed from the respective control group, p < 0.05.