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. 2017 Dec 19;9(1):1187–1199. doi: 10.18632/oncotarget.23455

Figure 9. GRM1+ exosomes induce anchorage independent growth in GRM1- cells.

Figure 9

C81-61 cells were stably transfected with either CD63-GFP or ptdTomato-CD81. The Keyence BZ-X710 florescent microscope was used to confirm the presence of GFP or ptdTomato florescent tags in stably transfected C8161 ptdTomato-CD81, C81-61 CD63-GFP, and C81-61 ptdTomato-CD81, the scale bar is 100μm (A). Cells were photographed after 21 days of growth in soft agar and exosomes. 1205Lu serves as a positive control. C81-61 CD63-GFP cells were plated in media with 0.33% agarose and incubated with either C8161 ptdTomato-CD81 exosomes that show significant number of colony formation, or with C81-61 ptdTomato-CD81 exosomes that only show two colonies (n=4, t-test, p=0. OT_031814_proof5), the scale bar is 100μm (B).