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. 2017 Oct 26;9(1):152–166. doi: 10.18632/oncotarget.22077

Figure 4. Deguelin inhibits VEGF production in HCC cells and suppresses VEGFR2 signaling pathway in HUVECs.

Figure 4

(A) and (B) Hep3B (A) and HepG2 (B) cells were treated with different concentrations of deguelin for 12 h. Cell culture media was harvested and VEGF level was measured by ELISA assay. (C) and (D) Hep3B (C) and HepG2 (D) cells were treated or not treated with 4 μM deguelin. Cell culture media was harvested at different time points and VEGF level was measured by ELISA assay. (E) Deguelin inhibits VEGF-induced VEGFR2 activation in HUVECs. HUVECs were starved in 0.5% FBS medium overnight and treated with various dosages of deguelin for 2 h, the cells were stimulated with VEGF (40 ng/ml) for 30 min. Cell lysates were then subjected to SDS-PAGE followed by Western blotting. β-Actin served as a loading control. (F) Inhibition of deguelin on VEGFR2 activation in a specific VEGFR2 inhibition assay. The experiment was conducted 3 times and the data are expressed as mean values ± S.D. (*p < 0.05, **p < 0.01, ***p < 0.001 versus untreated/vehicle group).