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. 2017 Dec 8;9(1):512–523. doi: 10.18632/oncotarget.23070

Figure 4. Antiproliferative effect of PAC-320 on prostate cancer cells involves apoptotic cell death.

Figure 4

(A) PAC-320 induces apoptosis in various human cancer cells. LNCaP or DU145 cells were treated with indicated concentrations of PAC-320 for 48 h. Cells were stained with Annexin V and PI and analyzed by FACS analysis. Representative flow cytometric dot plots showing an increased rate of apoptosis after PAC-320 treatment. (B) The data from three independent experiments in (A) are expressed as mean ± SD in the histogram. (C) PAC-320 induces caspase 3 and 7 activation and PARP cleavage. LNCaP or DU145 cells were treated with indicated concentrations of PAC-320 for 48 h. Whole cell lysates were immunoblotted with indicated antibodies. (D) PAC-320 induced caspase3/7 activities in LNCaP or DU145 cells. Cells incubated with indicated concentrations of PAC-320 for 48 h before caspase 3/7 activities were detected. The data from three independent experiments are expressed as mean ± SD in the histogram. *P < 0.05, **P < 0.01.