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. 2017 Dec 8;9(1):512–523. doi: 10.18632/oncotarget.23070

Figure 6. p38 MAPK signaling is involved in PAC-320 induced cell growth inhibition in DU145 cells.

Figure 6

(A) The effects of PAC-320 on MAPK phosphorylation. DU145 cells were treated with indicated concentrations of PAC-320 for 48 h, and whole cell lysates were immunoblotted with indicated antibodies. Numbers below the panels of the immunoblot indicated the protein levels relative to β-actin. (B) and (C) The effect of p38 MAPK inhibitor SB203580 on PAC-320 treatment effects. DU145 cells were treated with PAC-320 and/or 10 μM SB203580 for 48 h. The p38 phosphorylation or cell viability was determined by immunoblot (B) or MTT (C). Each value represents the mean of three experiments; bars, ± SD. *P < 0.05; **P < 0.01.