Table 1. The GPx2 KO influences the protein expression pattern in colonic tissue of mice.
| No | protein name | gene | Uniprot ID | mass (kDa) | pI | score | sequence coverage (%) | -Se WT | -Se KO | +Se WT | +Se KO | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell differentiation and proliferation | ||||||||||||||||
| 1 | Acidic leucine-rich nuclear phosphoprotein 32 family member B (AN32B) | Anp32b | Q9EST5 | 31.23 | 3.89 | 60 | 24 | 1.00 | 0.60# | 0.57* | 0.48 | |||||
| 2 | Transgelin-2 (TAGL2) | Tagln2 | Q9WVA4 | 22.55 | 8.39 | 58 | 46 | 1.00 | 1.20 | 0.52* | 0.53* | |||||
| 3 | Stathmin (STMN1) | Stmn1 | P54227 | 17.26 | 5.76 | 55 | 44 | 1.00 | 1.07 | 0.50* | 0.56* | |||||
| 4 | Calcium-activated chloride channel regulator 1 (CLCA1) | Clca1 | Q9D7Z6 | 100.81 | 5.67 | 63 | 22 | 1.00 | 0.59# | 1.34 | 1.18* | |||||
| 5 | Prelamin A/C (LMNA) | Lmna | P48678 | 74.48 | 6.54 | 62 | 27 | 1.00 | 0.75 | 1.61 | 1.17* | |||||
| 6 | Heat shock protein HSP 90-beta (HSP84) | Hsp90ab1 | P11499 | 83.57 | 4.97 | 95 | 22 | 1.00 | 0.87 | 1.40* | 1.14 | |||||
| Signal transduction | ||||||||||||||||
| 7 | Protein kinase C gamma type (PKCγ) | Prkcg | P63318 | 79.56 | 7.27 | 56 | 16 | 1.00 | 1.52# | 0.68* | 0.67* | |||||
| 8 | Dual specificity protein phosphatase 3 (DUS3) | Dusp3 | Q9D7X3 | 20.69 | 6.07 | 64 | 50 | 1.00 | 1.18 | 0.57* | 0.56* | |||||
| 9 | Calmodulin (CaM) | Calm1 | P62204 | 16.83 | 4.09 | 58 | 53 | 1.00 | 0.74 | 1.27 | 0.96# | |||||
| Cell metabolism | ||||||||||||||||
| 10 | Cytochrome c1, heme protein, mitochondrial (CY1) | Cyc1 | Q9D0M3 | 35.53 | 9.24 | 72 | 32 | 1.00 | 1.53# | 0.61 | 0.61* | |||||
| 11 | Cytochrome c1, heme protein, mitochondrial (CY1) | Cyc1 | Q9D0M3 | 35.53 | 9.24 | 84 | 35 | 1.00 | 1.34 | 0.16* | 0.19* | |||||
| 12 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (NDUS3) | Ndufs3 | Q9DCT2 | 30.3 | 6.67 | 60 | 22 | 1.00 | 1.22 | 0.58* | 0.61* | |||||
| 13 | Carbonyl reductase [NADPH] 3 (CBR3) | Cbr3 | Q8K354 | 31.33 | 6.15 | 85 | 39 | 1.00 | 1.07 | 0.53* | 0.54* | |||||
| Redox homeostasis | ||||||||||||||||
| 14 | Peroxiredoxin6 (PRDX6) | Prdx6 | O08709 | 24.97 | 5.71 | 72 | 38 | 1.00 | 1.36 | 0.65 | 0.79* | |||||
| 15 | Thioredoxin reductase 1, cytoplasmic (TR1) | Txnrd1 | Q9JMH6 | 68.24 | 7.42 | 62 | 22 | 1.00 | 1.08 | 1.51* | 1.39 | |||||
| Others (transport; metal ion binding; cytoskeleton organization) | ||||||||||||||||
| 16 | Transthyretin (TTHY) | Ttr | P07309 | 15.88 | 5.77 | 61 | 44 | 1.00 | 1.34# | 0.62* | 0.71* | |||||
| 17 | Cytosolic Fe-S cluster assembly factor NUBP2 (NBP 2) | Nubp2 | Q9R061 | 29.90 | 6.07 | 58 | 37 | 1.00 | 1.13 | 0.54* | 0.58* | |||||
| 18 | Tropomyosin alpha-1 chain (TPM1) | Tpm1 | P58771 | 32.72 | 4.69 | 80 | 29 | 1.00 | 0.93 | 1.26 | 1.45* | |||||
| 19 | Tropomyosin alpha-1 chain (TPM1) | Tpm1 | P58771 | 32.72 | 4.69 | 91 | 34 | 1.00 | 1.19 | 1.37 | 1.55 | |||||
Values are given as means (n = 4) in relation to the –Se WT group. Levels marked with # indicate significant differences between WT and KO within the same Se level and levels marked with * indicate significant differences between WT and KO versus the respective group maintained on –Se conditions. (p < 0.05; one-way ANOVA). +Se, selenite supplementation (150 µg Se/kg diet); score, Mascot Probability Based Scoring.