Table 1. Polymerase chain reaction primer pairs.
Gene | GenBank accession number | Primer sequence | Anneal (°C) |
---|---|---|---|
TH | NM_012740.3 | 5′ TTCTGGAACGGTACTGTGG 3′ 5′ AATCACGGGCGGACAGTA 3′ |
60 |
Nurr1 | NM_019328.3 | 5′ AGAGGGTGGGCAGAGAAGAT 3′ 5′ CTGGGTTGGACCTGTATGCT 3′ |
60 |
DAT | NM_012694.2 | 5′TCCTGAAAGGTGTGGGCT3′ 5′GAGCAGTTGGGGCTATTC3′ |
60 |
En1 | XM_001056699.6 | 5′ GGAGAAAGACTCGGACAGGT3′ 5′ GGTCGTAAGCAGTTTGGCTA 3′ |
60 |
Pitx3 | NM_019247.1 | 5′ GAATCGCTACCCCGACATGA 3′ 5′ TACACCTCCTCGTAGGGTGG 3′ |
60 |
Lmx1b | NM_053709.1 | 5′ TGCGGAAGGGTGATGAGTTC 3′ 5′ CCACTGCCCTTATTCTGGCT 3′ |
60 |
Nr1h3 | NM_031627.2 | 5′ TTCGTCCACAGAAGCGGAAA 3′ 5′ CGTGCTCCCTTGATGACACT 3′ |
60 |
Nr1h2 | NM_031626.1 | 5′ AGCGATCTTTCTCCGACCAG 3′ 5′ TTGGCGAAGTCCACGATCTC 3′ |
60 |
GAPDH | NM_017008.4 | 5′ ACAGCAACAGGGTGGTGGAC3′ 5′ TTTGAGGGTGCAGCGAACTT 3′ |
60 |